Verification of accuracy of qPCR method for intravital diagnostics of Macrorhabdus ornithogaster in avian droppings

The objective of this study was to verify the accuracy quantitative PCR (qPCR) method for in vivo detection Macrorhabdus ornithogaster. A total 100 faecal and gastric mucosa samples from avian cadavers were investigated, using cytological qPCR techniques (budgerigars, Fischer’s lovebirds, red-crowned parakeets, scarlet-chested parrots, eastern rosellas, domestic canaries, zebra finches, white Java sparrow). Using qPCR, probability detecting positive droppings significantly higher than smear microscopy (P < 0.01). Cytology detected presence ornithogaster 34 samples, whereas 54 samples. In all 46 negative performed also yielded results. Gastric 20 where has confirmed result. This verifies sample intravital diagnostics. Overall, technique appears be extremely reliable, as it made it possible detect infected individuals, including those with stool or cytology.